EXPLORING POTENTIAL OF METAL RESISTANT AND PGP ATTRIBUTES OF PHOSPHATE SOLUBILIZING RHIZOBACTERIA ISOLATED FROM SOUTHERN PUNJAB, PAKISTAN

Phosphorus is highly required nutrient for proper plant functioning and growth but due to its reactive nature it changed form after short period of time when we applied as fertilizer become unavailable uptake while Phosphate solubilizing rhizobacteria are powerful candidates that solubilize the P already present in soil. So, research based on hypothesis isolating, screening optimizing phosphate with potential metalresistant multiple PGPR attributes. Total 55 PSR strains were isolated out which 20 (WUSB1,WUSB2, WUSB3, WUSB4, WUSB5, WUSB6, WUSB7, WUSB8, WUSB9, WUSB10, WUSB11, WUSB12, WUSB13, WUSB14, WUSB15, WUSB16, WUSB17, WUSB18, WUSB19, WUSB20) selected 4-8 (PSI) 30-122?g/ml solubilization NBRIP medium one weekincubation at 370C. These further analyzed their physiochemical attributes, metal resistant, extracellular enzyme production PGP Final results showed candidate designated name WUSB16 produce significant i.e. having capacity 5.46 agar plate assay 105?g/ml broth assay, Acid alkaline phosphatase 20?mol PNPb ml-1 hr-1 or 15?mol PNP respectively. It resistant against ZnSO4, MnSO4 CuSO4. Evaluation attributes indicated strain auxin was 100?g/ml, siderophore nitrogen fixation stronger (+++), Hydrogen Cyanide ammonia (++) ACC deaminase negative (-). From above can conclude multifaceted traits best have utilized bioinoculant.